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Figure 2

Circular map comparing the Mel genome with the Ana, Sim and Moj assemblies. Ring 1 (outermost ring): forward strand genes; ring 2: reverse strand genes; ring 3: GC-skew plot; ring 4: X analysis of trinucleotide composition, with peaks indicating atypical regions; ring 5: Mel genes present in wAna assembly; ring 6: Mel genes present in the wSim assembly; ring 7: Mel genes present in Moj assembly. Large regions on the Mel genome that were not recovered in the Ana or Sim assemblies are marked on the outside (regions A, B).

Region A includes the 3'-region of the WO-A phage and the region directly downstream. It includes the interval containing genes WD0289-WD0296, which encodes four hypothetical proteins - three ankyrin repeat domain proteins and a conserved hypothetical protein. The absence of WD0289-WD0292 is interesting because it may suggest some variation in the phage 3'-region. Although WD0289-WD00291 is unique to WO-A, a protein homologous to WD0292 has been found in the previously described Wolbachia phage [ Womens Onlmanta Choker S/L Short WVN Dress Only Quality Free Shipping Outlet m2YkN
, 11 ]). Variation in the Wolbachia phage could facilitate the introduction of novel genes [ Womens Th Ath Ebony Tank Top Blue Midnight 16 Manufacturer Size XXLarge Tommy Hilfiger Cheap Explore Clearance Really cePGtVe
]. As ankyrin repeat proteins, WD0291, WD0292, and WD0294 are all of interest as they have been proposed to be involved in host-interaction functions [ Halston Heritage Woman Chiffonpaneled Cutout Knitted Sweater Pastel Yellow Size XL Halston Heritage Cheap Sale Manchester Great Sale Buy Cheap Classic Cheap Low Cost DbwVPezJ6
]. This could provide a means by which the phage could cause different host-interaction phenotypes.

Region B includes WD0509-WD0514, which encodes a DNA mismatch repair protein MutL-2, a degenerate ribonuclease, a conserved hypothetical protein, two hypothetical proteins and an ankyrin repeat domain protein. This region is of further interest since WD0511-WD0514 is found only in W. pipientis w Mel and not the related sequenced Anaplasmataceae, Rickettsiaceae or α-Proteobacteria. In W. pipientis w Mel, this region is flanked on the 3'-end by an interrupted reverse transcriptase and an IS5 transposase, supporting the hypothesis that it was acquired horizontally. The absence of MutL-2 might not be functionally important since w Mel, w Ana, and w Sim all have a copy of MutL-1.

Table 2

Percent identity between nucleotide sequences of the four sequenced strains of

As the table shows, the two most closely related strains are w Ana and w Sim, which are nearly identical at the DNA level. Both w Mel and w Moj are approximately equidistant from these two strains, at just over 97% identity, but are more distant from one another. Note however that because the w Moj sequences are single reads (that is, single-pass sequencing), the error rate in these sequences is substantially higher than in the assembled genomes of the other strains, which in turn may make it appear that w Moj is more divergent.

Nick Halla, chief strategy officer of Impossible Foods, faced some tough questions recently at the Sustainable Foods Summit
By Ken Roseboro
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An executive from a company selling a genetically engineered meat alternative faced tough questions at the Sustainable Foods Summit held in San Francisco at the end of January.

Nick Halla, chief strategy officer of Impossible Foods, gave a presentation about his company’s Impossible Burger as a sustainable solution to the problems of industrial meat production. He claimed their lab-created burger uses about 74 percent less water, generates about 87 percent fewer greenhouse gases and requires around 95 percent less land than conventional ground beef from cows. Halla said the Impossible Burger is seeing rapid acceptance in the marketplace, sold in many restaurants and “better burger” chains.

Doubts about Impossible Burger’s safety

But Halla’s PowerPoint slides didn’t mention that the Impossible Burger’s key ingredient is a genetically engineered protein called soy leghemoglobin or “heme.” The presentation also didn’t mention that the U.S. Food and Drug Administration told Impossible Foods that the company hadn’t demonstrated the safety of heme after it applied to the FDA seeking GRAS (generally recognized as safe) status. Despite FDA’s concerns, Impossible Foods sold its GMO-derived burger for public consumption anyway.

Several audience members took Halla to task over Impossible Foods marketing its burger despite FDA concerns, short-term feeding studies, and lack of transparency about the use of the GMO ingredient.

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, said he read the FDA documents about Impossible Foods application for GRAS status and was “shocked that a company could come out with a new food additive and not have it subjected to government and long-term scrutiny.”

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, asked Halla why his company had conducted such short, 14- and 28-day rat feeding studies of the product.

“Why did you do such short feeding studies when you know the minimum industry standard is 90 days?”

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Being in a revelation state: Whenever you are feeling an extremely pleasant or very deep sadness state, characterize a revelation state of being. You should never make decisions based on whatever you said or committed yourself that you will do while being in a revelation state. They are merely declarations made out of extreme emotions rather than results of calm, well focused thinking . The best recommendation is to never make a negative decision in the low time. Never make your most important decisions when you are in your worst moods. Wait. Be patient. The storm will pass.

Further Readings: Arsham H., Decidofobia: Miedo a la toma de decisiones importantes. ¿Cómo evitar tomar decisiones importantes?, Revista Inter-Forum , 16(3), 50-62, 2002. De La Boétie E., The Politics of Obedience: The Discourse of Voluntary Servitude , Harry Kurz (Translator), Black Rose Books Ltd., 1998. Gracian B., The Art of Worldly Wisdom: A Pocket Oracle , Currency Publisher, New York, 1992, Translation and Introduction by Ch. Maurer. This is a collection of aphorisms and reflections on the art of success in both private and public decision making. Leach J., Survival Psychology , Macmillian, 1994. Meerloo, J., The Rape of the Mind , The University Library, 1961. Rosen M., On Voluntary Servitude: False Consciousness and the Theory of Ideology , Harvard University Press., 1996. Sargant W., Battle For The Mind , Heinemann, London, 1976. Stephens L., and G. Graham, When Self-Consciousness Breaks: Alien Voices and Inserted Thoughts , MIT Press, 2000. Examines thought insertion as example of what the authors call "alienated self-consciousness." Sun-Tzu, M Missoni Woman Mini Red Size 40 M Missoni Free Shipping Cheap Real RUoXQ2
, R. Sawyer (Translator), Barnes and Noble Company, 1994. Sun-Tzu, and G. Michaelson, Sun Tzu: The Art of War for Managers , Pressmark International, 1999.

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Decision Problems or Decision Opportunities: At one time or another, organizations develop an over-abundance of decision problems. Sometimes they can be linked to organizational trauma, like down sizing, budget restraints or workload increases, but sometimes they evolve over time with no apparent triggering event. Increased complaining, a focus on reasons why things can't be done, and what seems to be a lack of active role characterize the "problem" organization. If the manager is walking negative and talking in a negative way, staff will follow.

In many instances we forget to find positives. When an employee makes an impractical solution, we are quick to dismiss the idea. We should be identifying the effort while gently discussing the idea. Look for small victories, and talk about them. Turning a problem into an opportunity is a result of many little actions . Provide positive recognition as soon as you find out about good performance. Do not couple positive strokes with suggestions for improvement. Separate them. Combining them devalues the recognition for many people. It is easy to get caught in the general complaining and bitching, particularly in customers' complains.

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